Our Services
Message
Research & Education are the Pillars of a Knowledge-based Sustainable Economy, Donate & Support us to help spread it to the lowest strata of society to create a brighter tomorrow for everyone- Team, BREF.
SERVICES OFFERED
Our Training & Courses
To higher secondary school, graduate and master students and Research Fellows
01. TRAINING & ASSISTANCE
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Summer Training Program – 2 months
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B.Sc course project work
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Support M.Sc project work in course synopsis preparation & experimentations
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Support Ph.D work in course synopsis preparation & experimentations
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Help in manuscript preparation and submission
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Special Training Program in Virology and Microbiology
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Animal handling and experimentations
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Online teaching, training and assistance for B.Sc, M.Sc and Ph.D. student
02. Hands on Experience for proper handling of different Biotechnological Instruments and Experiments.
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37°C Incubator
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Pipetting techniques (handling of pipettes including Pipettes)
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ELISA Plate Reader
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PCR work-station
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Conventional Thermal Cycler- PCR Machine
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Real-time PCR (RT-PCR)
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Gel electrophoretic apparatus to rum DNA & RNA samples
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Protein electrophoresis equipment & power supply
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Gel Documentation System
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Staining of agarose and polyacrylamide gel staining visualization
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Preparation of LB media
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Preparation of LB Agar plate
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Streaking techniques
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Bacterial culture in LB agar plate
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Cooling Centrifuge
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Mini-spin centrifuge
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Normal Centrifuge
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PH meter
03. SAMPLE COLLECTION for DNA, RNA, PROTEIN EXTRACTION
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Collection of samples from blood, water, urine, saliva etc.
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DNA extraction and analysis
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RNA extraction and analysis
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Protein extraction and analysis
04. IMMUNOSTAINING & IMMUNOBLOT- THEORETICAL TRAINING COURSES
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Hematoxylin and Eosin (H&E) staining
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Sample preparation for Immunohistochemistry (IHC)
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Theoretical demonstration of Immunohistochemistry (IHC) techniques
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Theoretical demonstration of Immunofluorescence (IF) techniques
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Western Blot
05. ANIMAL CELL CULTURE - TRAINING COURSES
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Freezing and Thawing of cells
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Media preparation
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Passaging of cells
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Transfection
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DNA, RNA & Protein extraction from cell culture
06. RECOMBINANT DNA TECHNOLOGY - TRAINING COURSES
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Sub-cloning and Cloning of viral, bacterial and human genes
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Plasmid DNA isolation
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Agarose gel electrophoresis
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Restriction digestion followed by agarose gel electrophoresis
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Ligation followed by agarose gel electrophoresis
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DNA extraction from agarose gel
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Preparation of competent cells
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Plasmid transformation in competent cells and plating on LBA plates
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Restriction digestion to confirm the size of insert
07. BIOINFORMATICS - TRAINING COURSES
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Bioinformatics of DNA databases
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Sequence analysis, Pairwise sequence alignment
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Multiple sequence alignment
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BLAST (Sequence homology)
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Designing of PCR Primers
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Restriction Analysis, Restriction maps, Plasmid maps
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Phylogenetic analysis of different microbial analysis especially virus
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Designing of site directed mutagenesis primers
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Analysis of cloned sequences
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Analyzing RNA sequences (promoter analysis)
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Analyzing Protein sequences (ORF sequence and start and stop codon)
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Simple Statistical Tools - Excel Based
Diagnostic Testing Services
01. BLOOD TESTS
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Blood typing
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Blood sugar
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Blood pressure
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Liver function test
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Kidney function test
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Complete blood count (CBC) - tests
02. RADIOLOGICAL TESTS
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X-ray
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Ultrasound
03. MOLECULAR DIAGNOSTIC TESTS
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Viral testing – Through ELISA, PCR & RT-PCR
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Bacterial testing- Through ELISA, PCR & RT-PCR and Culture
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Genetic biomarkers for different diseases- ELISA, PCR & RT-PCR
Epidemiological Study
The presence of viral, bacterial or other microbial parasites in distinct geographical location.
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Typhoid
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Rotavirus
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Hepatitis E virus (HEV)
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Hepatitis A virus (HAV)
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Swine flu
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Influenza
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Parainfluenza
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Respiratory syncytial virus (RSV)
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Avian flu
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Common cold
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Dengue
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Meningitis
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Polio
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Japanese encephalitis virus
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Rabies
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Yellow fever virus
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Helicobacter pylori (H. Pylori)
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Tuberculosis
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Malaria
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Unknown etiology
Consultancy Services
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Viral infectious diseases and treatment options
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Bacterial infectious diseases and treatment options
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Gastric & Liver Cancer- Prevention and Treatment options
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Gastrointestinal Motility
Counseling Services
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HIV/AIDS awareness and counseling program
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Women empowerment and socio-economic development
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Child welfare and education
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Old age home facility
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Counseling of drug addicts
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Gender resource center
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Vaccine awareness and vaccination camp
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Environment Protection
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Plantation, Cleanliness drive
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Carbon emission control
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Green & Clean Energy awareness
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Free ambulance service for poor people
How To Find Us
Call Today
07463092414